Composite

Part:BBa_K5250024:Design

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-10-01)


Constitutive promoter, T7-like RNAP + pT7-like, GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2715
    Illegal BamHI site found at 3510
    Illegal BamHI site found at 4390
    Illegal BamHI site found at 5270
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2898
    Illegal AgeI site found at 3359
    Illegal AgeI site found at 4251
    Illegal AgeI site found at 4363
    Illegal AgeI site found at 5131
    Illegal AgeI site found at 5243
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 156
    Illegal BsaI site found at 1220
    Illegal BsaI site found at 2090
    Illegal BsaI.rc site found at 108
    Illegal BsaI.rc site found at 600
    Illegal BsaI.rc site found at 1528
    Illegal BsaI.rc site found at 2538
    Illegal SapI site found at 2519
    Illegal SapI.rc site found at 1714


Design Notes

The sequences are codon optimized.


Source

The sequences for the T7-like RNAP and promoter were taken from a Pseudomonas phage Pf-10 gene bank. The rest of the sequences are from the iGEM Parts Registry.

References